Direct-write assembly of 3d hydrogel scaffolds for guided cell growth

Moderate mechanical properties for structural support Rapid degradation [10] Silk Silk fibroin protein from Bombyx mori, the silkworm, is one of the most widely used bioinks due to its biocompatibility, robust mechanical strength, controllable degradability, and minimal inflammatory response [ 46 ]. Because of these various advantages, tissues such as cartilage, bone, and fat have been cell printed with silk fibroin bioink [ 47 - 49 ].

Self-folding polyhedra and microdevices We are a highly interdisciplinary group that develops new methods to fabricate very small devices and integrated structures, and characterize these systems using microscopy and spectroscopy. A major thrust of our research is focused on constructing miniaturized 3D devices which are especially challenging to fabricate at small size scales.

Chengzhi Hu - Department teacher - SUSTC Department of Mechanical and Energy Engineering

Here, we have pioneered a variety of self-folding and self-assembly approaches, most notably for self-folding patterned polyhedra, folded metamaterials and actuators. We are also particularly interested in understanding, synthesizing and characterizing self-assembling, intelligent and hybrid biotic-abiotic systems.

We utilize a range of experimental techniques including photo- e-beam and nano-imprint lithography; 3D printing, thin film deposition, molding, etching, culture of prokaryotic E coli and eukaryotic e. It is very challenging to manufacture 3D micro and nanostructures in a cost-effective manner.

Conventional micro and nanopatterning is inherently 2D or layered 2D to 3D. Bend, curve and fold photo, e-beam and nanoimprint lithographically patterned thin films composed of device grade silicon, metals, inorganics, polymers and hydrogels.

Nanomanufacturing of patterned 3D nanostructures, anatomical models for tissue engineering, metamaterials in a high throughput and cost-effective manner Prior accomplishments in this research theme a.

High-throughput parallel nanomanufacturing of 3D nanostructures by bending, folding and curving of lithographically patterned thin films. We were the first laboratory to demonstrate the self-folding of nm sized curved and polyhedral metallic and dielectric structures with 10 nm resolved patterns in all three dimensions.

This demonstration opens the door to the creation of precisely lithographically patterned nanoparticles and nanostructures in a highly parallel and possibly cost-effective manner with nanoimprint lithography.

Self-assembly of lithographically patterned nanoparticlesNano Lett. High throughput folding using surface forces capillary origami We invented a high-throughput methodology to create well sealed and precisely patterned hollow polyhedra with metals, semiconductors and polymers.

Importantly, the work involves the invention see patents of self-aligning hinges that self-align and correct defects during folding. Self-aligning or liquid hinges can be used to seal and self-correct origami structures formed by any method.

Consequently, we have been able to fold polyhedra with angles as precise as Moreover, on cooling, the polyhedra are well sealed and mechanically rigid.

Fabrication of micrometer-scale, patterned polyhedra by self-assemblyAdv. Mater ; Self-assembled three-dimensional radio frequency RF shielded containers for cell encapsulationBiomed.

High throughput self-folding of containers, sheets and metamaterials using thin film stress We were the first laboratory to demonstrate geometrically programmable self-folding sheets and metamaterials composed of lithographically patterned metals and polymers. Sheets feature hundreds to thousands of folds, and fold up without any human intervention, wires or circuits.

By merely, varying the pattern of hinges on a lattice of rigid segments, we showed in Adv Matersee Figure 2 that it was possible to program the sheet to self-fold into a variety of different outcomes.

Programming a sheet to self-fold into different outcomes is an important feature for programmable matter. Subsequent demonstrations highlighted bidirectional curvature for mechanical metamaterials APL,and extension to truly reversible polymer electromagnetic metamaterials based on swelling Nature Comm Patterning thin film mechanical properties to drive assembly of complex 3D structuresAdv.

From nanopolyhedra to graphene origamiMRS Bull. High throughput parallel self-folding of biomimetic and anatomically inspired structures Tissues are highly curved, folded, patterned and vascularized.

We were the first laboratory to demonstrate the self-folding of microfluidic networks, synthetic scaffolds and cell-laden hydrogels reminiscent of vascularized leaves and tissues. Importantly, self-folding approaches enable layering, patterning and vascularization which are all important features of native tissues.

Directed growth of fibroblasts into three dimensional micropatterned geometries via self-assembling scaffoldsBiomater.

direct-write assembly of 3d hydrogel scaffolds for guided cell growth

Stimuli responsive self-folding with polymers and hydrogels We have worked extensively of stimuli responsive self-folding and demonstrated self-folding of metallic and hydrogel devices and actuators in response to a range of stimuli including pH, ionic strength, biochemicals, temperature and light.

Importantly we demonstrated Venus fly trap like actuators, the first ever variants of Miura-ori Nojima-ori reversible folding stimuli responsive EM metamaterials Nature Commun aboveporous gel based actuators or theragrippers and other functional devices.

To our knowledge we were the first to demonstrate photopatterning of cross-linking to modulate folding vertically, laterally and through gradients. Photolithographically patterned smart hydrogel based bilayer actuatorsPolymer ; Laser triggered sequential folding of microstructuresAppl.

Self-folding thermo-magnetically responsive soft-microgrippersACS Appl. Interfaces ; Self-folding graphene polymer bilayer s, Appl. Self-folding of functional devices: Sensors, optical, electronic, RF and lab-on-a-chip devices We have shown how self-folding can be utilized to create a variety of 3D optoelectronic and sensory devices.

Remote radio frequency controlled nanoliter chemistry and chemical delivery on substratesAngew. Like the classic saddle shape of a potato chip, self-folding of curved 2D shapes display a rich phase behavior including buckling or helical assembly.

direct-write assembly of 3d hydrogel scaffolds for guided cell growth

Plastic deformation drives wrinkling, saddling and wedging of annular bilayer nanostructuresNano Lett.Dec 09,  · Using other ink designs, such as those based on silk fibroin, hydrogel and fugitive organic inks, we have constructed 3D scaffolds and microvascular networks for tissue engineering and cell culture via direct-write assembly 8 BEGINNING-TO-END SOLUTIONS FOR STEM CELL RESEARCH Optimize the cell culture environment to direct cell differentiation and specialization with Corning’s tools and technologies.

1. Corning Extracellular Matrices: Choose from a wide variety of animal- or human-derived biological ECMs as well as synthetic ECM options for 2D and 3D stem cell differentiation. A polymer scaffold was first created by direct-write assembly, followed by the conformal growth of oxide and semiconductor layers, and removal of the polymer and oxide (foreground), as reported on p.

by Paul Braun, Jennifer Lewis, and co-workers. 3-Dimensional Scaffold-Tissue Construct Bioprinting Chemical Engineering B April 23, demonstrated that a fibrin gel scaffold promotes in vitro angiogenesis when seeded with hepatocytes and ECs. With the ability to create a patterned formation of cells in 3D bioprinting, an ideal structure can be designed.

PuraMatrix: Self-assembling Peptide Nanofiber Scaffolds The three-dimensional assembly of cells in a biological microenvironment is designed to recapitulate normal tissue function (1).

Often this involves the use of biomaterials. The successful 3-D cell growth. cell bio ch STUDY. Structure and support for the cell - Scaffold - allowing cells to take on a variety of shapes Filamin crosslinks actin filaments into a 3D-like gel network - Such networks are important underlying structures of membrane projections cells use to crawl across surfaces.

Gracias Laboratory. JHU.